Delivery of Antisense Oligonucleotides to the Cornea
نویسندگان
چکیده
منابع مشابه
Polymersome delivery of siRNA and antisense oligonucleotides.
siRNA and antisense oligonucleotides, AON, have similar size and negative charge and are often packaged for in vitro delivery with cationic lipids or polymers-but exposed positive charge is problematic in vivo. Here we demonstrate loading and functional delivery of RNAi and AON with non-ionic, nano-transforming polymersomes. These degradable carriers are taken up passively by cultured cells aft...
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Oligonucleotides (ODNs) do not readily get access to their desired site (cytoplasm/nucleus) of action due to their high hydrophility and high molecular weight. Therefore enhancing components (carriers) are needed. Most widely used in vitro are cationic lipids (liposomes). Other systems include peptide conjugates or complexes, polymers and nanoparticles. We used cationic lipids and polymers, wit...
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Antisense oligonucleotides efficiently inhibit gene expression in vitro; however, the successful therapeutic application of this technology in vivo will require the development of improved delivery systems. In this report we describe a technique that efficiently delivers antisense oligonucleotides into cells using molecular conjugates. This technique, which was initially developed for the deliv...
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Synthetic nucleic acids have shown great potential in the treatment of various diseases. Nevertheless, the selective delivery to a target tissue has proved challenging. The coupling of nucleic acids to targeting peptides, proteins, and antibodies has been explored as an approach for their selective tissue delivery. Nevertheless, the preparation of covalently coupled peptides and proteins that c...
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Therapeutic oligonucleotides, such as splice switching ONs (SSOs), provide opportunities for treating serious, life-threatening diseases. However, the development of ONs as therapeutic agents has progressed slowly, because difficult cytosolic delivery of SSOs into the cytosol and nucleus remains a major barrier. Photochemical internalization (PCI), a promising strategy for endosomal escape, was...
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ژورنال
عنوان ژورنال: Nucleic Acid Therapeutics
سال: 2020
ISSN: 2159-3337,2159-3345
DOI: 10.1089/nat.2019.0838